Job Details
Senior Scientist, Flow Cytometry
Job Description
General Summary:
Vertex Cell and Gene Therapy (VCGT) is seeking a Senior Scientist with strong hands-on experience developing, qualifying, running, and teaching flow cytometry. This role will establish a flow cytometry core facility at VCGT, providing support for a cross-functional group of scientists across multiple innovative programs from discovery through development. The candidate must thrive in a fast-paced environment, be able to troubleshoot and teach, and be committed to developing high-quality flow cytometry and cell sorting experiments.
Key Duties and Responsibilities:
- Support and train a cross-functional group of scientists on flow cytometry instrument use, panel design, sample acquisition, troubleshooting, analysis, and interpretation
- Design and optimize high throughput, high dimensional cell-based assays (phenotypical and functional) with multi-parametric flow-based readouts to support early and late-stage research projects
- Perform cell sorting experiments and train users on cell sorting techniques
- Maintain and troubleshoot instruments, including daily QC
- Advise stakeholders on flow cytometry assay best practices and technical details
- Stay abreast of flow cytometry innovations and provide guidance on cytometer purchases
Knowledge and Skills:
- 5+ years of experience operating flow cytometers and sorters, including previous experience working in a flow cytometry core lab or shared resource laboratory
- Operating expertise with BD flow cytometers and cell sorters including FACSymphony A3 and A5, FACSCelesta, FACSLyric, LSRFortessa X-20, and FACSAria; Life Technology Attune NxT; Sony sorters including MA900, Beckman Coulter MoFlo Astrios sorter, and/or other flow/FACS instrument experience
- Experience with Amnis Imagestream imaging-based cytometers
- Experience with Miltenyi AutoMACS and MACS-based sorting techniques
- Expertise with BD FACSDiva, FlowJo and other flow analysis software
- Experience characterizing primary human samples, including hematopoietic stem cells, immune cell subsets, beta cells, and/or induced-pluripotent stem cells
- Experience developing and conducting flow cytometry-based readouts for primary human effector assays
- Experience designing, running, and analyzing high-dimensional flow panels (10+ colors)
Education and Experience:
- Ph.D. (or equivalent degree) in molecular and cell biology, developmental biology, stem cell biology, or related disciplines and 2-5 years of relevant post-doctoral employment experience, or
- Masters Degree (or equivalent degree) in molecular and cell biology, developmental biology, stem cell biology, or related disciplines and 7+ years of relevant employment experience, or
- Bachelors Degree (or equivalent degree) in molecular and cell biology, developmental biology, stem cell biology, or related disciplines and 9+ years of relevant employment experience
Company Information
Vertex is a global biotechnology company that invests in scientific innovation.
Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, sexual orientation, marital status, or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.
Please note that Vertex requires COVID-19 vaccination for all employees working in the U.S. as a condition of employment at Vertex, subject to medical, religious or other accommodations or exemptions required by law. We believe this requirement is vital to keeping everyone at Vertex and in our communities safe. This requirement applies to both existing and new employees who may at any time work on-site at a Vertex facility or visit another location on behalf of Vertex (e.g., conferences, customer visits, etc.), including all remote employees. Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager, or contact Talent Acquisition at [Register to View]